An RNA Aptamer Specific to Hsp70-ATP Conformation Inhibits its ATPase Activity Independent of Hsp40
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چکیده
منابع مشابه
DnaJA1/Hsp40 is co-opted by influenza A virus to enhance its viral RNA polymerase activity.
UNLABELLED The RNA-dependent RNA polymerase (RdRp) of influenza A virus is a heterotrimeric complex composed of the PB1, PB2, and PA subunits. The interplay between host factors and the three subunits of the RdRp is critical to enable viral RNA synthesis to occur in the nuclei of infected cells. In this study, we newly identified host factor DnaJA1, a member of the type I DnaJ/Hsp40 family, act...
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The molecular chaperone and cytoprotective activities of the Hsp70 and Hsp40 chaperones represent therapeutic targets for human diseases such as cancer and those that arise from defects in protein folding; however, very few Hsp70 and no Hsp40 modulators have been described. Using an assay for ATP hydrolysis, we identified and screened small molecules with structural similarity to 15-deoxyspergu...
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DbpA is a putative Escherichia coli ATP dependent RNA helicase belonging to the family of DEAD box proteins. It hydrolyzes ATP in the presence of 23S ribosomal RNA and 93 bases in the peptidyl transferase center of 23S rRNA are sufficient to trigger 100% of the ATPase activity of DbpA. In the present study we characterized the ATPase and RNA unwinding activities of DbpA in more detail. We repor...
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ژورنال
عنوان ژورنال: Nucleic Acid Therapeutics
سال: 2015
ISSN: 2159-3337,2159-3345
DOI: 10.1089/nat.2014.0510